The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Remove the plate seal and read on an HTRF compatible reader. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Your lab might even have one already. Incubate overnight at RT. • HTRF®-Certified Reader. certified for use with HTRF. For more information about HTRF. Small ONE- PLATE ASSAY PROTOCOL: overnight FOR HTRF CERTIFIED READER . Plate 25 μL of cells in. Incubate 4h at RT. It measures 96-well plates in less than 20 seconds and 1536-well formats in under one minute. Cover the plate with a plate sealer. It guarantees high level of cAMP accumulation in the cells. The plate was covered and incubated for one hour at room temperature. Incubate overnight at RT. Read & analyse. As shown in Fig. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Place the imaging filter cube on top of the LED cube you installed, and use the hex wrench to screw it to the LED cube. If not, our scientific team will help you choose the instrument that best suits your needs and budget. You can count on our plate readers to bring you the performance, reliability, consistent results, and the best user experience available—across a multitude of applications and a range of detection options. For multiplexed luminescent and fluorescent assays, using a white plate will support maximum light output signal for the luminescent portion of the assay, but result in higher crosstalk and background for the fluorescent portion of the assay. 96-well culture plateFOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. Plate 25 μL of cells in. Plate 25 μL of cells in. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. Customer Transformation Hub. The dual measurement. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. 96-well culture plateONE-PLATE ASSAY PROTOCOL: Small volume detection plate. " The PHERAstar FSX instrument is a high quality, reliable, plate reader. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. 5. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. FOR HTRF CERTIFIED. The kit includes everything you need: reagents, buffers and a microplate. PLATE READER INTRODUCTION Selecting the right plate reader for your lab can be challenging. Lysates are then transferred to the assay plate for the detection of total-EGFR by HTRF. Lysates are then transferred to the assay plate for the detection of total-NFkB by HTRF. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Small volume detection plate 96-half well plate 25 µL cells + 5 µL compounds + 10 µL supplemented lysis buffer (4X)* + 4 µL HTRF® pre-mixed antibodies Read & analyse Incubate 4h at RT Transfer 16 µL supernatant LysisAdd ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified . Revvity. For more. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the market with all major detection technologies including Alpha and certification for HTRF® technology plus software designed for. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the. 1. SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. You can verify your reader’s capacity to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. The 2 plate protocol involves culturing cells in a 96-well plate before lysis then transferring lysates to a 384-well low volume detection plate before adding Phospho-ERK (Thr202/Tyr204) HTRF detection reagents. → Incubate 2h (if the detection is not sensitive enough, it is better to incubate 20h). Part Number# Product Description Number of Plates# 66PL96001 HTRF 96 well low volume plate 1 plate 66PL96005 HTRF 96 well low volume plate 5 platesThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. These plates can be read on all HTRF certified readers, thus making their implementation in. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations. FOR HTRF CERTIFIED READER For more. Manufacturer. FOR HTRF CERTIFIED. 1) . Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. For more information about HTRF. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. It can also be further streamlined toLysates are then transferred ®to the assay plate for the detection of Total TYK2 by HTRF reagents. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. 96-well culture plateONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. For more information about HTRF. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Plus . PROTOCOL AT A GLANCE Do not pre-mix the d2 and Cryptate solutions prior to dispensing. Plate cells Stimulate cells 3. Finding a plate reader that offers compatibility with microplates sold by multiple leading vendors can protect both the long-term cost and the performance of assays. These plates can be read on all HTRF certified readers, thus making their implementation in. After an overnight incubation, the plate is washed 3 times. For more information about microplate recommendations, please visit our website • Store the kit at -16°C. Plate 25 μL of cells in. These plates can be read on all HTRF certified readers, thus making their implementation in. FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. As shown in Fig. Read & analyse. Remove the plate sealer and read on an HTRF® compatible reader . VICTOR® Nivo™ multimode plate reader 2 | For research use only. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Smallest and most affordable multimode plate reader in the industry. This protocol gives the cells viability and confluence to be monitored. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. FOR HTRF CERTIFIED READER. Plate 25 μL of cells in. Plate 25 μL of cells in. Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. FOR HTRF CERTIFIED READER. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. HTRF Signal + 2 hours @ RT [Cortisol] ng/ml 10 µL Standard or Sample 5 µL Cortisol acceptor reagent 5 µL Cortisol donor antibody ADD READ & ANALYSESafire 2™ readers must be appropriately be configured for HTRF readout by setting up the measurement conditions in the “multilabeling” function of Magellan software. First the plate is coated with a capture antibody. For more information about HTRF. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Transcreener® ADP2 FP assay. Incubate at room temperature. 5. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. pre+ 5 µL HTRFcompounds Activation Buffer. Lysates are then transferred to the assay plate for the detection of total cRaf by HTRF. Bench top solutions for cell based assays. HTRF is compatible with a large range of available microplate readers. For HTRF certified reader For more information about HTRF® compatible readers and for set- up recommendations, please visit our website. The detection antibody is incubated for 1. An example of a LanthaScreen kinase assay run on a BMG LABTECH plate reader can be seen in the application note " LanthaScreen TR-FRET tyrosine kinase and protein kinase C assay ". These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. Seal the plate and. For more ® information about HTRF compatible readers and for set-up recommendations, please. Plate Type and Read Area should be selected based on the microplate used and wells to be read. Typical applications The HTRF cartridge provides optimal performance for key applications. (donor) of compounds are measured on an HTRF-compatible reader. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. 25 µL cells + 5 µL compounds + 10 µL supplemented lysis buffer (4X)* + 4 µL HTRF ® pre-mixed antibodies. Synergy NEO2 readers must be appropriately configured for HTRF® readout by setting up the measurement conditions in the Gen5™ Reader Control and Data Analysis Software. Its. For more information about HTRF ®• HTRF®-Certified Reader. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Even if not listed, some readers may be compatible with HTRF technology. Revvity. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. → Cover the plate with a plate sealer. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. Microplate readerFluorescence microplate readers usually contain a light source, an optical system for the selection of excitation light (i. </p> <p>And, the Enhanced Security software option includes tools to facilitate 21 CFR Part 11 compliance for integration into regulated environments (GxP). Optimization of the reader for the plate dimensions clearly improved the results as seen by the uniform signal (b), a lowered %CV of 2. Time-resolved fluorescence was measured switch the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). Plate 25 μL of cells in. Incubate 4h at room temperature. Fluorescence intensity – Fluorescence intensity is one of the most popular detection modes, and there are innumerable fluorophores available for applications such as DNA or protein quantitation, reporter-geneCertified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. FOR HTRF CERTIFIED READER. Make sure to use the set-up for Eu Cryptate. The signals are produced by, converted by, or transmitted through the. These plates can be read on all HTRF certified readers, thus making their implementation in. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. The DLR assay is a two step reaction that uses two luciferase enzymes, Firefly and Renilla (Figure 1). For the standard curve described, all wells of the microplate are read. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. FOR HTRF CERTIFIED. Flexibility combined with the best sensitivity of its class, and our newly developed Enhanced Dynamic Range technology, make it the ideal reader for assay development. It features a modular design that allows users to add or change detection cartridges according to their needs. The ratio between control lysate signal / non-specific signal should be greater than 2. Read & analyse. FOR HTRF CERTIFIED. FOR HTRF CERTIFIED READER. Plate Reader Requirement. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Read & analyse. FOR HTRF CERTIFIED READER. Make sure the setup for Eu3+ Cryptate is used. CLARIOstar is equipped with a specific optical device which enables the measurement of. Handle complex biological matrices easily. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. DLReady™ certified luminometer plate readers. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. As shown in Fig. For more information. ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. EnVision Nexus ultimode Plate Reader 2 Benefits • Provides the speed and sensitivity to boost your lab’s productivity with ultra-high throughput • Freely configurable and upgradable to adapt to your needs today and tomorrow • Fully automatable for walkaway convenience • Certified for use with HTRF® technology Key features BMG LABTECH plate readers certified for LanthaScreen detection include the PHERAstar FSX, CLARIOstar Plus and VANTAstar. Read & analyse. e. No washing steps are required. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Page 30 4. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Other technologies such as imaging. For more information about HTRF. Nephelometers are dedicated instruments that can only detect scattering and are only available as standalone plate readers. Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. Up to 8 decades dynamic range in one measurement with EDR technology. After defining the objectives and their locations in Gen5: 1. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. FOR HTRF CERTIFIED READER. Plate 25 μL of cells in. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. EX: HTRF Emission range. FOR HTRF CERTIFIED READER. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4 SpectraMax® M5 Multi-Mode Microplate Reader MLDVM5 SpectraMax® M5e Multi-Mode Microplate Reader (HTRF certified) MLDVM5E To prevent evaporation during assay incubation steps, we recommend the use of TopSeal-A PLUS, a clear adhesive seal for microplate. CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified . COMPONENTS ; STORAGE . Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. It comes equipped with our patented LVF Monochromators™, filters, and spectrometer. For more information about HTRF ®Detection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. For more information about HTRF ® compatible readers and for set-up recommendations, please. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. At Cisbio we provide microplates that help you discover better. GENERAL LAB WORK USING HTRF KIT 1 Plate 3 mL of cells in 6-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. FOR HTRF CERTIFIED READER. The VICTOR Nivo. Homogeneous Time-Resolved Fluorescence (HTRF) • Certified for detection of Cisbio’s HTRF assays • GPCR assays (cAMP cell based, membrane based) • Kinases and ATPases • Cytokine Assays TUNE Multi-Mode • cAMP Quantitation • Cell Migration • Cell Viability and Proliferation • Cytotoxicity • DNA/RNA Quantitation (Fluorescent) Homogeneous Time-Resolved Fluorescence (HTRF) . Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. Already more than 50 certified or compatible instruments, and counting! HTRF is compatible with a large range of available microplate readers and certainly the detection system already in your lab. 4 mg/ml. The SpectraMax i3x and iD5 readers can be equipped with an HTRF-certified detection cartridge or an HTRF-certified detection system, respectively. $1,024. View Brochure. com HTRF Microplate Readers. For more information about HTRF. HTRF Signal + 5 hours @ RT or . For instance, when assay volumes are. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Remove the plate seal and read on an HTRF compatible reader. Lysates are then transferred to the assay plate for the detection of total-p53 by HTRF. It is used as a positive control for Gi applications ( biological models validation,FOR HTRF CERTIFIED READER. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:320/40 HTRF Eu cryptate 665/7uv Wide Filter 0. No washing steps are required. Partnerships for delivering turn-key solutions. HTRF Microplate Readers. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. The CulturPlate is PerkinElmer’s microplate designed for cellular assays, tissue-culture treated and sterilized by gamma-irradiation to facilitate adherent cell growth. Make sure the setup for Tb Cryptate is used. Signals are detected at two different wavelengths (typically 620 nm and 665 nm), which are used to. 5 hours before 3 more washing steps. CLARIOstar is equipped with a specific optical device which enables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. On BMG LABTECH´s NEPHELOstar Plus, a laser diode (at 635 nm) with a highly collimated beam is used as high-intensity light source. White 384-well low volume plate Make sure to use the set-up for Eu 3+ Cryptate. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. Results for HTRF Reader Control Kit with black and white microplates. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. For more ® information about HTRF compatible readers and for set-up recommendations, please. The assay was prepared in both white and black plates and incubated >18 hours. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERDetection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. 1) . . The measurement of HTRF assays is predominantly performed on microplate readers. This protocol, HTS designed, allows miniaturization while. EM1 616/10 nm EM2 665/10 nm Certified for use with Cisbio’s HTRF Europium Cryptate reagents. Step 5 Remove the plate sealer and read on an HTRF® compatible reader The blank control is used to check the Cryptate signal at 620 nm. overnight at 4 C [Prostaglandin E2] pg/ml. + 4 µL HTRF pre-mixed antibodies. e. The optional HTRF-certified filter-based module may be. Fluorescence intensity – Fluorescence intensity is one of the most. For more information about HTRF. The Dual-Luciferase ® Reporter Assay or DLR is widely used to study gene transcription and regulation. Remove the plate sealer and read on an HTRF® compatible reader . HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Add ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. 0. HTRF® Europium cryptate donor / Red acceptor readout Setup recommendations for Tristar 5FOR HTRF CERTIFIED READER. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Lysis buffer 1X for assay protocol on tissues, two-plate assay protocol on adherent cells, and standard dilution. Conclusion. For more information about HTRF. Both readers demonstrated their ability to detect IP1 concentrations according to Cisbio’s acceptance criteria. FOR HTRF CERTIFIED READER. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option. For more information about microplate recommendations, please visit our website • Store the kit at -16°C. Interaction with the industry and research communities is essential for innovating! For more than 30 years, Cisbio has been serving the needs of the clinical and drug discovery communities, delivering a world-class portfolio of assays designed to help find answers faster. These plates can be read on all HTRF certified readers, thus making their implementation in. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The LVis Plate contains 16 spots which can be loaded with 2 microliters of sample for subsequent absorbance measurements. FOR HTRF CERTIFIED READER. Plate 25 μL of cells in. The EnSight<sup>®</sup> multimode plate reader offers HTS Alpha and Ultra-sensitive Luminescence technologies alongside conventional multimode detection technologies. 96-well culture plateThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The HTRF cellular assays were found to be at least 16-fold more sensitive than the Western Blot. Lysates are then transferred to the assay plate for the detection of Total Cofilin by HTRF® reagents. These convert photons generated by fluorescence or. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. HTRF 96-well low volume plate *To prepare working standard solutions, culture medium can be an alternative the diluent. FOR HTRF CERTIFIED READER. Read & analyse. FOR HTRF CERTIFIED READER. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. Read & analyse. The system provides even higher sensitivity for time-resolved fluorescence (TRF) applications. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. The kit includes everything you need: reagents, buffers and a microplate. Read & analyse. It also integrates with the. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. FOR HTRF CERTIFIED READER. 5 µL Prostaglandin E2 . HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). Read & analyse. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. Microplate Readers and Reader Accessories. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READERThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. But sometimes these parameters are not sufficient enough. FOR HTRF CERTIFIED READER. The Z´ value takes into account four (4) parameters: the means (µ) and the standard deviations (σ) of the positive (plateau) and the negative (baseline) controls 1. It can also be further streamlined to a one-. Its. Detection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Alpha Technology: The VICTOR Nivo system. at room temperature STEP 4 Remove the plate sealer and read on an HTRF ® compatible reader. Note that any dilution or improper use of d2 and cryptate Tb-detection reagents will impair the assay quality. FOR HTRF CERTIFIED READER. Whatever the plate type, assay volume should match well volume as closely as possible so that focalization of the reader excitation beam gives maximum energy delivery (Fig. FOR HTRF CERTIFIED READER For. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:FOR HTRF CERTIFIED READER. 96-well culture plateThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same . 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non-specific short-lived emissions. Both readers demonstrated their ability to perform the cAMP G s HiRange assay with results well within Cisbio’s required. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Reader. The kit includes everything you need: reagents, buffers and a microplate. FOR HTRF CERTIFIED READER. Transcreener® ADP2 TR-FRET assay. PHERAstar FSX Powerful and most sensitive HTS plate reader . FOR HTRF CERTIFIED READER . Suggested plate map: 1: 2 3: A: 14 μL Std 0: 3 μL IP1 d2 Reagent 3 μL IP1 Tb Cryptate Antibody• HTRF®-Certified Reader. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYFOR ®READING, AN HTRF -CERTIFIED READER IS NEEDED. use of a wide variety of plates or sample matrix formats. FOR HTRF CERTIFIED READER. These plates can be read on all HTRF certified readers, thus making their implementation in. Make sure the setup for Eu Cryptate is used. • HTRF®-Certified Reader **. Concentrate on running your assays instead of running your instrument: the optic modules contain all the filters, dichroics, and more needed to run your assay. Make sure the setup for Tb Cryptate is used. Incubate overnight at RT. Make sure the setup for Tb Cryptate is used. It can also be further streamlined toFOR HTRF CERTIFIED READER. Make sure the setup for Tb Cryptate is used. The samples were each placed in a well of a 24-well plate, covered with 1 ml of culture medium, and incubated for a total of 17 days. -CERTIFIED READER IS NEEDED. This protocol gives the cells viability and confluence to be monitored. This is thanks to a dedicated Alpha Technology laser which outperforms any xenon-based light source by exciting donors at 680 nm. FOR HTRF CERTIFIED READER. . Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. FOR HTRF CERTIFIED READER. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). At its core, Synergy H1 is a monochromator-based multi-mode microplate reader with a quadruple grating architecture for robust performance and flexibility. For more information about HTRF. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. PerkinElmer. Cells in each image are identified by SoftMax Pro Software and cell-by-cell HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. This protocol enables the cells' viability and confluence to be monitored. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERHTRF Ratio Anti-Tag Antibody Cryptate Inhibitor-Red Inhibitor Purified Tagged Kinase Figure 2: Principle of HTRF kinase competition binding assay (IC 50 - KI determination) HTRF Dasatinib-Red Part # 62KB02REDC & 62KB02REDE Amount: 1 nmol (62KB02REDC) & 20 nmol (62KB02REDE) Concentration: 25 µM in DMSO Form:. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody blockers in a high throughput format. These are the different kinetic modes on BMG LABTECH microplate readers. Make sure the setup for Eu 3+ Cryptate is used. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readers HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. For more information about HTRF ®Seal the plate and incubate for Overnight. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. HTRF-compatible SpectraMax Multi-Mode Readers. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Make sure the setup for Tb Cryptate is used. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. 3-in-1 absorbance using a variety of sample formats, including microplates. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. The Bradford assay was successfully performed on the BMG LABTECH microplate reader. DATA REDUCTION & INTERPRETATION 1. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. No washing steps are required. Your lab might even have one already. Incubate overnight at RT. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. FOR HTRF CERTIFIED READER For more information about HTRF. Lysates are then transferred to the assay plate for the detection of total cRaf by HTRF. Lysates are then transferred to the assay plate for the detection of total-CREB by HTRF. EnVision Nexus ultimode Plate Reader 2 Benefits • Provides the speed and sensitivity to boost your lab’s productivity with ultra-high throughput • Freely configurable and upgradable to adapt to your needs today and tomorrow • Fully automatable for walkaway convenience • Certified for use with HTRF® technology Key featuresBMG LABTECH plate readers certified for LanthaScreen detection include the PHERAstar FSX, CLARIOstar Plus and VANTAstar.